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Subsequently, a 200-μl fixation buffer was utilized to deal with the cells for 20 min. Soon after washing the cells three times with precooled PBS, one× intracellular staining permeabilization clean buffer made up of 1% DAPI (blue) was applied to mark the nuclei from the cells. Ten minutes later on, the https://caidendowgn.answerblogs.com/26614231/not-known-factual-statements-about-12-dehydrogingerdione


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