As Vp was preset and E0 didn't alter, channel conductance should have lessened. Also, in the outside-out patches, an analogous gradual reduction in conductance was recorded. It's not necessarily inconceivable that bicuculline, when it binds on the GABA-binding web site, could change the conformation on the channel. A part of https://emilianorxcjm.jaiblogs.com/55824812/an-unbiased-view-of-iwr-1